Nexcelom Video Library
Introduction to Cellometer K2
PC-based instrument for automated 4x bright field and dual channel fluorescent cell counting, viability, intensity, and population analysis for hepatocytes, stem cells, splenocytes, tumor suspension and other primary cells.
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Transcript: Cellometer K2 Image Cytometer for Cell Counting and Analysis
The Cellometer K2 is an Image Cytometer for Cell Counting and Analysis.
The system provides consistent, reliable and accurate counting results for primary cells.
Viability can be done with either trypan blue or fluorescent stains.
Cell-based assays such as Cell Cycle or Apoptosis can also be performed.
Here is an example of a viability measurement. 20 micro liters of PMBC sample is mixed with Acridine Orange and Propidium Iodide solution and then loaded into the Cellometer counting chamber.
Unlike manual counting with a hemacytometer, the counting chamber is loaded into the Cellometer K2 which replaces the users eyes and microscope.
Cell images are previewed and a click of a button starts cell counting.
Fluorescent stained live and dead cells produce strong signals among debris and unwanted cell types.
Cellometer takes cell images and analyzes them based on preset parameters to determine live and dead cell concentration and viability.
After cell images are acquired, results are displayed on the screen.
Results include the total count, cell size histogram, percent viability and concentration.
Results can be printed or saved to a network location in Text or Excel file formats.
To meet desired target cell concentration or cell number, the software includes an intuitive sample adjustment calculator.
Cell images can be viewed to confirm counted cells.
The all plastic counting chamber is easily disposed of after use.
The Cellometer K2 consistently delivers reliable and accurate results. Cellometer, Image Cytometry