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Cellometer Spectrum

Image Cytometry System for 20 µl Cell-Based Assays

Cellometer Spectrum Image Cytometer

Cell Cycle

Apoptosis

Cell-Mediated Cytotoxicity

Cell Counting and Viability

Interchangeable Fluorescent Modules

Features of the Spectrum Image Cytometry System

Whether you are looking for an affordable benchtop cytometer, or to improve your current Flow cytometry analysis, the Spectrum Image cytometry system will exceed your expectations not only in performance, data analysis and affordability, but also in its ease of use and application versatility.

All-in-One System

Basic cell counting, primary cell viability, and cell-based assays.

Dual-Fluorescence for Accurate Primary Cell Viability

No interference from red blood cells. Analyze bone marrow, peripheral blood, and cord blood without lysing.

Unique Algorithms for Advanced Cell Analysis

Determine concentration and viability of hepatocytes, adipocytes, and other sophisticated cell types.

Fast Results

Obtain cell images, counts, size measurements and viability calculations in < 30 seconds per sample.

Simple 20 µl Cell-Based Assays

  • Pre-qualified reagents
  • Small 20 µl sample size
  • Simple, image-based analysis
  • Pre-defined instrument settings
  • Assay-specific data templates
  • Accurate, consistent results
Simple Cell-Based Assays

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Cell Types for Many Research Areas

Cellometers are optimized for primary cell analysis

PBMCs
Stem Cells
Adipocytes
Neural Cells
Hepatocytes
Dendritic Cells

Epithelial Cells
Keratinocytes
Lymphocytes
Splenocytes
Monocytes

  • Clinical Immunology: PBMCs
  • Diabetes / Obesity: Adipocytes
  • Immunotherapy: Leukocytes
  • Microbiology: Yeast (Spectrum 10x)
  • Oncology: Cell Lines
  • Regenerative Medicine: Stem Cells
  • Toxicology: Hepatocytes
  • Transplantation: Nucleated Cells
  • Vaccine Development: Splenocytes

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Advantages of Cellometer Image Cytometry

Cell Imaging

  • Visually check cell morphology
  • Ensure only cells of interest are counted
  • Archive and re-analyze cell images
  • Export images for publication

Proprietary Pattern-Recognition Software

  • Count individual cells in clusters
  • Count irregular-shaped cells
  • Count cells based on size
  • Eliminate debris from cell counts

Non-Fluidic Platform

  • Disposable counting chambers – no washing
  • Compatible with fragile cells
  • Maintenance-free
  • Robust optics modules and LED light sources

IQ/OQ Validation and GMP/GLP Accessories

  • Installation Qualification reagents/protocol
  • Operational Qualification reagents/protocol
  • On-site IQ or OQ Performance
  • GMP/GLP Software Module

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Accurate Cell Counting

Bright Field

Bright field cell image

Stained AO+ Cells

Stained AO + Cells

Counted AO+ Cells

Counted AO + Cells
Cellometer Spectrum Dynamic Range
  • Automatic identification of Acridine Orange (AO) stained cells (shown above)
  • Image and count up to 1.51x107 cells/mL
  • Image acquisition and analysis: 30 seconds/sample for a 3-channel assay
  • Versatile platform - use for routine cell counting and complex cell-based assays

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Export to FCS Express* for Flow-Like Data

Counted PI+ Cells

Counted PI + Cells

Contour Population Plot

Contour population plot
  • Export image data into flow cytometry software: FCS Express
  • Simple work flow: No fluid- stream, No PMT voltages, No forward/side scatter
  • Easily perform data analysis using pre-designed templates
  • Quickly plot cell population data as a: histogram, scatter plot, dot plot or contour plot

Propidium Iodide (PI) Cell Cycle

PI Cell cycle data

* FCS Express Flow Cytometry software is a product of De Novo Software and is included with the Cellometer Spectrum

Cell Population % of Gated Cells Concentration
(10^6 cells/mL)
Total 100 3.18
Sub G1 3.8 0.12
G0/G1 61.9 1.97
S 15.3 0.49
G2/M 19 0.60

Histogram of PE CD3+ Jurkat cells

Histogram of PE CD3+ Jurkat cells

CD3 Bright Field

CD3 Bright Field

CD3 PE

CD3 PE

Contour map of Jurkat cells, showing GFP+ and RFP+ intensity profiles.

Contour map of Jurkat cells

Bright Field

Bright Field

GFP/RFP Positive Cells

GFP/RFP Positive Cells

Proven Results

  • Determine cell viability, for cell lines or primary samples, using AO/PI in seconds
  • Accurately measure cell samples with varying viability (0 – 100%)
Measured Jurkat Cell Viability

Bright Field

Bright Field

Stained AO+ Cells

Stained AO+ Cells

Stained PI+ Cells

Stained PI+ Cells

Combined Counted Cells

Combined Counted Cells

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User-Changeable Fluorescence Optics Modules*

Cellometer assays use specific optic modules for maximum performance and discrimination between fluorescence channels. Example filter modules are listed below. Additional filter modules available upon request. Please check our website for the most recent modules available.

Optics Module Fluorophores Nucleic Acid Stains
  S1-534-470

Ex: 470nm (452-498nm)

Em: 534nm (510-560nm)

GFP

Calcein

FITC

AlexaFluor® 488

AO (acridine orange)

SYTO®9, SYTO®13

SYTOX®Green

SYTO®BC

  S1-605-527

Ex: 527nm (498-550nm)

Em: 605nm (570-638nm)

AlexaFluor® 546

AlexaFluor® 555

Cy3®

PE (R-phycoerythrin)

RFP

PI (propidium iodide)

EB (ethidium bromide)

SYTOX® Orange

  S1-655-527

Ex: 527nm (525-558nm)

Em: 655nm (642-665nm)

RFP

7-AAD

Nile Red

PI (propidium iodide)

EB (ethidium bromide)

*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.
Sytox, AlexaFluor, and Cy are trademarks of Life Technologies.

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