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Cellometer X2 Applications

Platelets

Platelets

Automatically measure mouse and human platelet concentration using brightfield only method. »

Yeast Viability

Yeast Concentration & Viability

Live yeast cell concentration and viability are measured automatically based on the membrane permeability of the fluorescent dye Oxonol. Multiple strains of yeast with viability ranging from 88 to 5% were measured. »

Performance of the Cellometer X2 Image Cytometer

Dynamic Range of Total Cells with Cellometer X2

Dynamic Range of Total Cells with X2

Figure 1: Table of results for cell concentration dynamic range

Concentration Dynamic Range Figure 1 depicts the dynamic range for cell concentration measured by Cellometer X2. This data set was taken on a concentration series of cultured yeasts.

Samples from 2.5 x 105 – 5 x 107 cells/ml can be counted without further dilution.

The %CV at each concentration was below 10%.

Cellometer X2 Repeatability and Consistency

X2 Average Live Cell Concentration Via Fluorescence Viability
AVE 1.32E+07 78.1%
STDEV 7.69E+05 2.2%
CV (%) 5.84 2.78

Figure 2: Table of results for cell concentration and viability using acridine orange (AO) and propidium Iodide (PI)

The results indicate the accuracy of the Cellometer X2 instrument in assessing the viability of yeasts using AOPI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X2 in measuring cell concentration and viability of yeast cells.

Performance of the Cellometer X1 Image Cytometer

Dynamic Range of Total Cells with Cellometer X1

Dynamic Range of Total Cells with X1

Figure 3: Table of results for cell concentration dynamic range

Concentration Dynamic Range Figure 3 depicts the dynamic range for cell concentration measured by Cellometer X1. This data set was taken on a concentration series of cultured yeasts.

Samples from 4 x 105 – 5 x 107 cells/ml can be counted without further dilution.

The %CV at each concentration was below 10%.

Cellometer X1 Repeatability and Consistency

X1 Average Live Cell Concentration Via Bright Field Viability
AVE

4.07E+07

69.0%

STDEV

2.28E+06

2.5%

CV (%)

5.61

3.64

Figure 4: Table of results for cell concentration and viability using bright-field and Propidium iodide (PI)

The results indicate the accuracy of the Cellometer X1 instrument in assessing the viability of yeasts using PI for cell viability. Yeasts were tested at 24 sample replications. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer X1 in measuring cell concentration and viability of yeast cells.

Cellometer X1 and X2 Consistency and Accuracy Comparison to Hemacytometer

5 µm beads

N = 20 Hemacytometer X1 & X2
Average 1.07E+06 1.10E+06
STDEV 5.90E+04 9.81E+04
%CV 5.5% 8.9%