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Cellometer Auto T4 Cell Counter: More Than 1,600 Counted Cell Lines

Cellometer Auto T4 Applications

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »


Cell Image Gallery Visit the Cellometer Cell Image Gallery for a More Complete Listing

Cancer Cell Lines Counted with the Cellometer Auto T4

Cell Characteristics are based on observation in a Cellometer Counting Chamber. For adherent cell lines, cell characteristics are based on the appearance of cells following trypsinization.

786-O

786-0 cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

A498

A498 cells

Origin: Kidney

Cell Characteristics: Round, some size variation

A549/ATCC

A549/ATCC cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, clusters > 5 cells

ACHN

ACHN cells

Origin: Kidney

Cell Characteristics: Mostly round, normal size distribution, clusters < 5 cells, debris

CAKI-1

CAKI-1 cells

Origin: Kidney

Cell Characteristics: Large variation in morphology

CCRF-CEM

CCRF-CEM cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

COLO 205

COLO 205 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution

DU145

DU145 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

EKVX

EKVX cells

Origin: Leukemia

Cell Characteristics: Round cells, variable size distribution, clusters < 5 cells

HL-60

HL-60 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

HOP-62

HOP-62 cells

Origin: Lung, NSCLC

Cell Characteristics: Round cells, some size variation, cell clusters < 5 cells

HOP-92

HOP-92 cells

Origin: Lung: NSCLC

Cell Characteristics: Round cells, some size variation

HS 578T

HS 578T cells

Origin: Breast

Cell Characteristics: Large variation in morphology, debris

HT-29

HT-29 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

K562

K562 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

LOX IMVI

LOX IMVI cells

Origin: Skin

Cell Characteristics: Large variation in morphology, debris

M-14

M-14 cells

Origin: Skin

Cell Characteristics: Round, some variation in size

MALME-3M

MALME-3M cells

Origin: Skin

Cell Characteristics: Round, normal size distribution

MDA-MB-231/ATCC

MDA-MB-231/ATCC cells

Origin: Breast

Cell Characteristics: Round, some size variation, some debris

MDA-MB-435

MDA-MB-435 cells

Origin: Breast

Cell Characteristics: Round, some size variation

MDA-MB-468

MDA-MB-468 cells

Origin: Breast

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

MOLT-4

MOLT-4 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

NCI/ADR-RES

NCI/ADR-RES cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution

NCI-H226

NCI-H226 cells

Origin: Lung, NSCLC

Cell Characteristics: Round, some size variation, some clusters < 5 cells

NCI-H23

NCI-H23 cells

Origin: Lung NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

NCI-H322M

NCI-H322M cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

OVCAR-4

OVCAR-4 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells, some debris

OVCAR-5

OVCAR-5 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells

OVCAR-8

OVCAR-8 cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

PC-3

PC-3 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution

RPMI 8226

RPMI 8226 cells

Origin: Leukemia

Cell Characteristics: Round, size variation

SF-268

SF-268 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SF-295

SF-295 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SF-539

SF-539 cells

Origin: Brain

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

SK-MEL-2

SK-MEL-2 cells

Origin: Skin

Cell Characteristics: Large variation in morphology and size

SK-MEL-5

SK-MEL-5 cells

Origin: Skin

Cell Characteristics: Variation in morphology and size

SK-MEL-28

SK-MEL-28

Origin: Skin

Cell Characteristics: Variation in morphology and size

SK-OV-3

SK-OV-3 cells

Origin: Ovary

Cell Characteristics: Variation in morphology and size, some clusters < 5 cells

SN-12C

SN-12C cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

SNB-19

SNB-19 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SNB-75

SNB-75 cells

Origin: Brain

Cell Characteristics: Round, some variation in size and morphology

SR

SR cells

Origin: Lymphoma

Cell Characteristics: Large variation in morphology

SW620

SW620 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

T-47D

T-47D cells

Origin: Breast

Cell Characteristics: Large variation in morphology and size, some clusters < 5 cells

UACC-257

UACC-257 cells

Origin: Skin

Cell Characteristics: Large variation in size and morphology, some clusters < 5 cells

Performance of the Cellometer Auto T4 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Auto T4
Average 1.03E+06 1.08E+06
STDEV 6.60E+04 1.01E+05
%CV 6.4% 9.4%

5 µm beads

N = 20 Hemacytometer Auto T4
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 6.46E+04
%CV 5.5% 6.3%